Final
| PL | Athlete | Team | Final | HPL |
| 1 | Thomas, Brionna | Fort Wayne Express TC | 24.66 | 2(1) |
| 2 | Arnold, Dejah | Fort Wayne Express TC | 25.12 | 2(2) |
| 3 | Caudle, Maya | Indiana Storm TC | 25.57 | 2(3) |
| 4 | Jones, Briana | Indiana Storm TC | 26.18 | 2(4) |
| 5 | Harris, Ashanetta | Indys Racing Cheetahs | 26.55 | 1(1) |
| 6 | Hargrove, Breanna | Indiana Storm TC | 26.60 | 2(5) |
| 7 | Vandever, Devyn | Indiana Storm TC | 26.69 | 1(2) |
| 8 | Kelley, Kendall | Fort Wayne Express TC | 27.00 | 1(3) |
| 9 | Robinson, Kourtney | Indiana Storm TC | 27.20 | 2(6) |
| 10 | Davis, Alexa | Indys Racing Cheetahs | 27.72 | 1(4) |
| 11 | Garmon, Alecia | Invictus Track Field | 27.89 | 1(5) |
| 12 | Owens, Ebone | Indiana Storm TC | 28.82 | 1(6) |
| 13 | Thompson, Kaia | Carmel Stallions | 28.96 | 2(7) |
| Nelson, Ariane | Fort Wayne Express TC | DNS | 1() |
| Bostic, Alexis | Fort Wayne Express TC | DNS | 1() |
| Etgen, Cameron | Unattached | DNS | 2() |
Final Heat: 1
| PL | HPL | Athlete | Team | Final |
| 5 | 1 | Harris, Ashanetta | Indys Racing Cheetahs | 26.55 |
| 7 | 2 | Vandever, Devyn | Indiana Storm TC | 26.69 |
| 8 | 3 | Kelley, Kendall | Fort Wayne Express TC | 27.00 |
| 10 | 4 | Davis, Alexa | Indys Racing Cheetahs | 27.72 |
| 11 | 5 | Garmon, Alecia | Invictus Track Field | 27.89 |
| 12 | 6 | Owens, Ebone | Indiana Storm TC | 28.82 |
| | Nelson, Ariane | Fort Wayne Express TC | DNS |
| | Bostic, Alexis | Fort Wayne Express TC | DNS |
Final Heat: 2
| PL | HPL | Athlete | Team | Final |
| 1 | 1 | Thomas, Brionna | Fort Wayne Express TC | 24.66 |
| 2 | 2 | Arnold, Dejah | Fort Wayne Express TC | 25.12 |
| 3 | 3 | Caudle, Maya | Indiana Storm TC | 25.57 |
| 4 | 4 | Jones, Briana | Indiana Storm TC | 26.18 |
| 6 | 5 | Hargrove, Breanna | Indiana Storm TC | 26.60 |
| 9 | 6 | Robinson, Kourtney | Indiana Storm TC | 27.20 |
| 13 | 7 | Thompson, Kaia | Carmel Stallions | 28.96 |
| | Etgen, Cameron | Unattached | DNS |